Hydrocortisone-modulated Immune Response and Altered Amino Acid Pool in Vitro

Peter N. Uchakin, Scott M. Smith, Olga N. Uchakina


Background: Dietary deficiencies, from macro- to micronutrients, are often associated with dysfunction of the immune system. The aim for this study was to assess changes in amino acid metabolism and immune response in a modeled stress environment.

Methods: Heparinized blood samples from healthy donors were collected by venipuncture. Peripheral blood mononuclear cells were isolated and then incubated with a mixture of LPS and PHA in RPMI-1640 medium. Cell cultures were treated with hydrocortisone at physiological (10-8 M) and stress (10-6 M) concentrations. Surface expression of CD25 by the CD3+ lymphocytes was analyzed by flow cytometry. Levels of the cytokines IL-2, IFN-gamma, IL-4, and IL-10 were measured with ELISA. Quantification of IL-2- and IFN-gamma- secreting T cells was performed in cell cultures treated with PMA, ionomycin, and brefeldin A.

: The number of CD3+CD25+ cells was significantly lower in cultures treated with 10-6 M of hydrocortisone than in control cultures. Treatment of cell cultures with 10-8 M hydrocortisone significantly increased levels of IFN-gamma and IL-10, but not IL-2 and IL-4. Treatment with 10-6 M of hydrocortisone significantly suppressed secretion of all studied cytokines. Treatment with 10-8 M hydrocortisone produced a consistent unidirectional effect on amino acids in the supernatant medium: increased concentrations of almost all amino acids. Treatment with 10-6 M hydrocortisone significantly decreased the level of asparagine while it increased levels of serine and tyrosine compared to control cultures.

Conclusion: Data suggest that the stress-dose of cortisol modulated amino acid metabolism in mitogen-stimulated immunocompetent cells in vitro. Also, observed differences in amino acid concentrations between cultures suggest that supplementation with low doses of endocrine mediators may create a more physiological environment for culturing PBMCs.

J Endocrinol Metab. 2011;1(4):166-173
doi: https://doi.org/10.4021/jem49w


Immunity; Cytokines; Cortisol; Stress

Full Text: HTML PDF

Browse  Journals  


Journal of Clinical Medicine Research

Journal of Endocrinology and Metabolism

Journal of Clinical Gynecology and Obstetrics


World Journal of Oncology

Gastroenterology Research

Journal of Hematology


Journal of Medical Cases

Journal of Current Surgery

Clinical Infection and Immunity


Cardiology Research

World Journal of Nephrology and Urology

Cellular and Molecular Medicine Research


Journal of Neurology Research

International Journal of Clinical Pediatrics



Journal of Endocrinology and Metabolism, bimonthly, ISSN 1923-2861 (print), 1923-287X (online), published by Elmer Press Inc.                     
The content of this site is intended for health care professionals.
This is an open-access journal distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, which permits unrestricted
non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Creative Commons Attribution license (Attribution-NonCommercial 4.0 International CC-BY-NC 4.0)

This journal follows the International Committee of Medical Journal Editors (ICMJE) recommendations for manuscripts submitted to biomedical journals,
the Committee on Publication Ethics (COPE) guidelines, and the Principles of Transparency and Best Practice in Scholarly Publishing.

website: www.jofem.org   editorial contact: editor@jofem.org
Address: 9225 Leslie Street, Suite 201, Richmond Hill, Ontario, L4B 3H6, Canada

© Elmer Press Inc. All Rights Reserved.

Disclaimer: The views and opinions expressed in the published articles are those of the authors and do not necessarily reflect the views or opinions of the editors and Elmer Press Inc. This website is provided for medical research and informational purposes only and does not constitute any medical advice or professional services. The information provided in this journal should not be used for diagnosis and treatment, those seeking medical advice should always consult with a licensed physician.