Journal of Endocrinology and Metabolism, ISSN 1923-2861 print, 1923-287X online, Open Access
Article copyright, the authors; Journal compilation copyright, J Endocrinol Metab and Elmer Press Inc
Journal website http://www.jofem.org

Original Article

Volume 1, Number 2, June 2011, pages 79-86


Hypothalamic Dynamics of CD26 Protein in Obese and Fasted Rats

Figures

Figure 1.
Figure 1. CD26 immunoreactivity in coronal section of rat brain. Pia mater (highlighted) of control (A) and food deprived MSG (B) animals. 2.12 mm posterior to bregma. Bars = 30µm.
Figure 2.
Figure 2. CD26 immunoreactivity in coronal section of rat brain. Retrochiasmatic nuclei (highlighted) of control (A) and MSG (B) animals.1.44 mm posterior to bregma. Bars = 30 µm.

Tables

Table 1. Density of CD26-ir in the Hypothalamus of Normal Control (C), Normal Food Deprived (FD), Obese (MSG) and Obese Food Deprived (MSG-FD) Animals
 
RegionsCFDMSGMSG-FD
Values are mean ± SEM. Number of animals in the parentheses. A = absence of immunoreactivity. *P < 0.05, **P < 0.009 and ***P < 0.0001 compared to C (paired, two-sided Student’s t test). ArcNH: arcuate nucleus hypothalamic; DMH: dorsomedial hypothalamus; Pe: periventricular hypothalamic nucleus; PVN: paraventricular hypothalamic nucleus; RCh: retrochiasmatic hypothalamic nucleus; SON: supraoptic hypothalamic nucleus; PM: pia mater.#Reference of localization in mm posterior to the bregma [24].
ArcNH (1.71 - 4.36 mm)#306 ± 11.26 (3)490 ± 33.77** (3)144 ± 15.88** (3)292 ± 26.56 (3)
DMH (2.28 - 3.84 mm)#14 ± 0.88 (3)27 ± 1.45** (3)10 ± 1.15 (3)A (3)
Pe (1.32 - 3.48 mm)#63 ± 12.12 (3)134 ± 19.63* (3)52 ± 9.81 (3)144 ± 24.26* (3)
PM (1.32 - 4.36 mm)#425 ± 37.58 (3)58 ± 7.22*** (3)256 ± 20.71* (3)51 ± 5.19*** (3)
PVN (1.32 - 2.28 mm)#47 ± 4.91 (3)88 ± 6.93** (3)A (3)A (3)
RCh (1.32 - 2.52 mm)#12 ± 1.15 (3)41 ± 7.8* (3)138 ± 11.14*** (3)A (3)
SON (1.32 - 2.52 mm)#8 ± 1.45 (3)A (3)79 ± 1.45*** (3)A (3)

 

Table 2. Density of Fos-ir in the Hypothalamus of Normal Control (C), Normal Food Deprived (FD), Obese (MSG) and Obese Food Deprived (MSG-FD) Animals
 
RegionsCFDMSGMSG-FD
Values are mean ± SEM. Number of animals in the parentheses. A = absence of immunoreactivity. *P < 0.05, **P < 0.009 and ***P < 0.0006 compared to C (paired, two-sided Student’s t test). AH: anterior hypothalamus; ArcNH: arcuate hypothalamic nucleus; DMH: dorsomedial hypothalamus; Pe: periventricular hypothalamic nucleus; PVN: paraventricular hypothalamic nucleus; RCh: retrochiasmatic hypothalamic nucleus; SON: supraoptic hypothalamic nucleus; VMH: ventromedial hypothalamus. #Reference of localization in mm posterior to the bregma [24].
AH (1.32 – 2.82)#63 ± 11.83 (3)51 ± 9.52 (3)27 ± 4.91* (3)60 ± 6.64 (3)
ArcNH (1.71 – 4.36)#437 ± 66.11 (3)352 ± 31.46 (3)347 ± 61.77 (3)101 ± 23.67** (3)
DMH (2.28 – 3.84)#577 ± 111.14 (3)443 ± 69 (3)12 ± 2.31** (3)239 ± 41.28* (3)
Pe (1.32 – 3.48)#120 ± 16.45 (3)78 ± 4.91 (3)16 ± 1.15** (3)16 ± 2.03** (3)
PVN (1.32 – 2.28)#207 ± 16.16 (3)135 ± 14.14* (3)66 ± 7.50** (3)30 ± 4.61*** (3)
RCh (1.32 – 2.52)#155 ± 19.92 (3)65 ± 7.50* (3)22 ± 4.04** (3)66 ± 11.26* (3)
SON (1.32 – 2.52)#25 ± 2.60 (3)A (3)A (3)A (3)
VMH (1.72 – 3.36)#408 ± 79.67 (3)74 ± 12.70* (3)A (3)118 ± 21.94* (3)